Figure 2.

Cell type-specific expression achieved with Split Gal4. (A) The B_AG and B_SEG are groups of neurons in the ventral nerve cord (VNC) and subesophageal zone (SEZ), respectively, that express the hormone, Bursicon (left). A Burs^Gal4DBD hemidriver in combination with two distinct enhancer-trap Zip⁺-VP16AD hemidrivers can be used to individually target each group (right panels). (B,C) Split Gal4 parsing of medulla neurons. (B) Medulla neurons that receive input from photoreceptors are labeled by the ort-C1a-Gal4 driver (ort^C1a-G4, left). Three subpopulations of these neurons, Tm5a, Tm5c, and Tm20, with different projection patterns, are identified by an ort-C1a^Gal4DBD hemidriver used with different enhancer-trap Zip⁺-dVP16AD hemidrivers (right panels). (C) Schematic showing input and output patterns of Tm5a, Tm5c, and Tm20 neurons (names outlined in green). Panel (A) adapted from Luan et al. (2012); panels (B,C) from Melnattur et al. (2014).