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. 2020 Nov 9;14:582218. doi: 10.3389/fnana.2020.582218

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Copyright © 2020 Zaqout, Becker and Kaindl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dewaxing and heat antigen retrieval steps. (A) Twelve histological staining boxes are cleaned, labeled accordingly, and filled with the related solutions. (B) The paraffin section slides are distributed in staining racks. (C) After dewaxing, the slides are transferred to glass staining racks and then to glass staining containers (D) filled with the unmasking solution. (E) The container with the slides is microwaved for 3 min after boiling, cooled down to approximately 50°C using an ice box (F) and then microwaved again for 3 min.