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. 2020 Nov 9;14:582218. doi: 10.3389/fnana.2020.582218

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Copyright © 2020 Zaqout, Becker and Kaindl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Blocking step. (A) A microscope slide box is used to prepare a humid box by wetting tissue papers and placing them at the bottom of the microscopic slide box grooves. (B) The box is placed on a flat balanced surface. (C) The slides are placed horizontally, one next to the other, on the edges of the humid box. (D) Using a 1,000 μl pipette, 190 μl of 5% BSA is added to each slide and the pipette is slightly pressed more to create some air bubbles (E) on top of the solution. (F) The air bubbles are then touched with the side of a pipette tip and slid over the slides to distribute the solution above the sections evenly without touching the sections.