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. 2020 Nov 9;14:582218. doi: 10.3389/fnana.2020.582218

FIGURE 5.

FIGURE 5

Immunofluorescence staining for different mouse tissues using our protocol. (A) The somatosensory neocortical region of an adult brain section stained for neuronal nuclei (NeuN; mature neuron marker, arrowheads) and parvalbumin (PV; interneuron marker, arrows). (B) Postnatal 0 (P0) retina section stained for sex-determining region Y—box 2 (SOX2; stem cell marker, arrowheads) and anti-tubulin beta III isoform (Tuj1; early neuron marker, arrows). GCL, ganglion cell layer; NBL, neuroblastic layer. (C) Adult testis section showing seminiferous tubules stained for mouse vasa homolog (MVH; spermatogenic cell marker, arrowheads) and anti-phospho-histone H3 (pH3; mitotic cell marker, arrows). (D) Adult skeletal muscle section stained for laminin (basement membrane marker) and paired box protein-7 (PAX7; muscle precursor cell marker, arrows). Immunofluorescence images: scale bar, 100 μm. For more details on the antibodies used in this figure, please refer to Table 1.