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. 2020 Nov 9;8:564169. doi: 10.3389/fcell.2020.564169

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Copyright © 2020 Hofbauer, Ondracek, Mangold, Scherz, Nechvile, Seidl, Brostjan and Lang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stimulation of human coronary artery endothelial cells with NETs. hCAECs were stimulated with isolated NETs, dsDNA, and citH3 for 6 and 24 h. MCP-1 gene expression was assessed (A) on mRNA levels by qPCR after 6 h and is presented as fold change over an unstimulated control after normalization to the mean of two endogenous controls. (B) Release of MCP-1 was measured in cell supernatants by ELISA after 24 h of stimulation. Data are presented as mean ± SD. Experiments were repeated five times and analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01.