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. 2020 Nov 9;8:564169. doi: 10.3389/fcell.2020.564169

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Copyright © 2020 Hofbauer, Ondracek, Mangold, Scherz, Nechvile, Seidl, Brostjan and Lang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MCP-1 primes neutrophils for NET formation. (A) Leukocytes of healthy donors (n = 8) were treated with 500 pg/ml of MCP-1 for 2:30 h and analyzed by flow cytometry. Data are presented as percentage of CD66b-positive cells staining for citH3. Groups were compared by Wilcoxon matched-pairs signed rank test. (B) Neutrophils of healthy donors (n = 12) were pre-treated with 125, 250, and 500 pg/ml MCP-1 and then stimulated with 1.3 μM ionomycin (IM) to induce formation of NETs. Data are provided in percent of positive control and are presented as mean ± SD. Experiments were analyzed by repeated measures ANOVA followed by Dunnett’s multiple comparisons test using the group treated with 1.3 μM IM as single comparator. *p < 0.05, ***p < 0.001, ****p < 0.0001.