FIGURE 4.

Effect of MCP-1 on accumulation of fibrocytes and fibrocyte CCR2 expression at the culprit site. Fibrocyte counts in culprit and peripheral site blood, presented as influx ratio at the culprit site, and mean fluorescence intensity (MFI) of CCR2 expression were determined by flow cytometry; MCP-1 was measured by ELISA and presented as fold increase at the culprit site. (A) Correlation of relative fibrocyte count at the culprit site with MCP-1 fold increase (Spearman signed-rank test, r_s = 0.361, n = 47). (B) Correlation of fibrocyte CCR2 expression at the culprit site with MCP-1 fold increase (Spearman signed-rank test, r_s = –0.443, n = 26). (C) CCR2 receptor expression on fibrocytes of culprit site (n = 26), peripheral site (n = 26), 72 h follow-up (n = 14), and healthy control blood (n = 18). Statistical tests for respective pairwise comparisons, and corrected and uncorrected p values are provided in Supplementary Table S2. Data are presented as boxplots with whiskers defined according to Tukey. *p < 0.05, **p < 0.01.