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. 2020 Nov 9;8:564169. doi: 10.3389/fcell.2020.564169

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Copyright © 2020 Hofbauer, Ondracek, Mangold, Scherz, Nechvile, Seidl, Brostjan and Lang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of MCP-1 and NET stimulation on fibrocyte CCR2 expression in vivo and in vitro. (A) Culprit site CCR2 expression on fibrocytes is presented relative to peripheral CCR2 expression. (B) PBMCs of healthy donors were stimulated for 6 h with 0.125, 0.250, 0.500, and 5 ng/ml recombinant MCP-1 and analyzed for CCR2 expression by flow cytometry. Data are presented relative to the unstimulated control as mean ± SD. (C) PBMCs of healthy donors were stimulated for 6 h with NETs (500 ng/ml dsDNA content), MCP-1 (5 ng/ml), NETs and MCP-1, and neutrophil control supernatant (PMN-SN) to investigate the effect on CCR2 expression as mean fluorescence intensity (MFI). Data are presented as mean ± SD. Experiments were repeated with four different donors and analyzed by repeated measures one-way ANOVA followed by Tukey’s multiple comparisons test. **p < 0.01, ***p < 0.001, ****p < 0.0001.