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. 2020 Nov 9;8:564169. doi: 10.3389/fcell.2020.564169

FIGURE 6.

FIGURE 6

Influence of MCP-1 and NETs on monocyte and fibrocyte migration in vitro. (A) PBMCs were subjected to chemotaxis assays using cell culture inserts. MCP-1 (500 pg/ml), NETs (500 ng/ml dsDNA content), and neutrophil control supernatant (PMN-SN) were compared as chemoattractants (well). Chemotaxis was assessed by flow cytometry recording of (B) monocyte and (C) fibrocyte cell counts/μl. (D) NETs (500 ng/ml dsDNA content) and neutrophil control supernatant were investigated as inhibitors of MCP-1-mediated migration. Migrated cells were stained and counted by flow cytometry recording (E) monocytes and (F) fibrocytes/μl. Experiments were repeated with PBMCs and NETs of 10 healthy donors. Monocyte counts of graphs (B,E) were compared with Friedman test followed by Dunn’s multiple comparisons test, and fibrocyte counts of graphs (C,D) were analyzed by repeated measures ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.