Table 2.

Examples of novel compounds produced through combinatorial biosynthesis using transient expression in N. benthamiana.

Source of enzymes	Enzymes	Compound class	Obtained compounds	References
Tanacetum parthenium/Artemisia annua	Germacrene A synthase (GAS) Germacrene A oxidase (GAO) Costunolide synthase (COS) Parthenolide synthase (PTS) aldehyde Δ11(13) double bond reductase2 (DBR2)	Sesquiterpenoids	Costunolide and parthenolide derivatives	Kashkooli Beyraghdar et al., 2019
Oat, licorice, soy bean, barrel clover	β-amyrin synthase and β-amyrin-oxidizing P450s	Triterpenoids	β-amyrin derivatives	Reed et al., 2017
Various species	Class I/II diterpene synthases	Diterpenoids	Diverse diterpene skeletons	Andersen-Ranberg et al., 2016
Setaria italica, Sorghum bicolor, Lotus japonicus, Brassica rapa	Cytochrome P450s Glucosinolate biosynthetic genes	Isothiocyanate	Crucifalexins	Calgaro-Kozina et al., 2020