FIGURE 1.

hAMSC and hAEC decrease the proliferation of T24 cells when grown in direct co-culture for 24 and 48 h. T24 cells were cultured alone or in the presence of different gamma-irradiated cell types (hAMSC or hAEC, CFPAC-1, Saos-2, dermal fibroblast cells). Cultures were performed in both contact (A,B) and transwell settings (C,D) at T24:gamma-irradiated cell ratios of 1:1 and 1:4. After 24 h (A,C) or 48 h (B,D) T24 cell proliferation was assessed by [3H]-thymidine incorporation. Data are expressed as violin plot of more than four independent experiments for each condition, five to nine biological samples of hAEC and hAMSC were used. *p < 0.05, **p < 0.01; ***p < 0.001.