FIGURE 2.

hAM scaffolds change the growth dynamic of T24 cells. T24 cells were seeded on (A) the hAM epithelium (e-hAM), (B) denuded hAM (d-hAM) without hAM epithelium but preserved basal lamina, (C) hAM stroma (s-hAM) of inverted hAM, and (D) on the synthetic scaffold. When grown on the hAM scaffolds, T24 cells growth was restricted. The growth restriction effect was pronounced especially by the e-hAM scaffold, followed by s-hAM and d-hAM scaffolds, especially in the first 3 days of culture (E) (p < 0.001). After 3 weeks in culture, T24 cells grown on the e-hAM (F) and s-hAM (H) scaffolds formed a 1–2 layered epithelium and on the d-hAM scaffolds they formed a 1–3 layered epithelium (G). On the synthetic scaffolds T24 cells grew in 2–6 layers (I). Scale bars: 50 μm.