FIGURE 6.

Individual T24 cells grown on hAM scaffolds expressed E-cadherin and occludin. (A,A’,B,B’) Individual T24 cells grown on e-hAM scaffolds expressed E-cadherin and occludin mainly in the cytoplasm. (C,C’,D,D’) The T24 cells grown on d-AM scaffolds did not express E-cadherin, while individual T24 cells grown on the d-AM scaffolds did express occludin. (E,E’,F,F’) The expression of E-cadherin and occludin was most prominent in the individual T24 cells grown on s-hAM scaffolds, in which both proteins were mainly dispersed in the cytoplasm and in some cases occludin was expressed on borders between adjoining cells. (G,I) T24 cells grown on synthetic scaffolds do not express E-cadherin nor occludin. (H,J) The strong expression of E-cadherin and occludin in normal porcine urothelial cells. Images show T24 cells on hAM scaffolds and synthetic scaffolds after 3 weeks in culture and normal porcine urothelial (NPU) cells on synthetic scaffolds after 4 weeks in culture. Green – Occludin, E-cadherin; DAPI – nuclei. Scale bars: 20 μm.