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. Author manuscript; available in PMC: 2020 Nov 23.
Published in final edited form as: Biochem Pharmacol. 2019 Aug 14;169:113604. doi: 10.1016/j.bcp.2019.08.006

Figure 9. Transient silencing of CSE and CPOX exhibit opposite effects on mitochondrial respiration of cultured liver cells.

Figure 9.

Representative Western blot images demonstrate significant attenuation of (A) CSE and (B) CPOX protein expressions mediated by siRNA silencing approach. (C) Mitochondrial respiration of n=3 Seahorse ‘coupling’ assays. (D) Calculated AUC of key bioenergetic parameters such as basal respiration, ATP production, maximal respiration, and spare respiratory capacity. Note that CSE silencing significantly enhanced mitochondrial respiration in all parameters, whereas CPOX silencing worsened the calculated ATP production or maximal respiration values. Double silencing of CSE and CPOX overall improved the basal respiration, ATP production, and maximal respiration compared to vehicle. Data are shown as mean ± SEM of n=3 independent experiments. *p<0.05, **p<0.01 CSE−/− vs. siCTL group or CSE−/−/siCPOX vs. siCPOX group; #p<0.05, ##p<0.01 siCPOX vs. siCTL group.