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. Author manuscript; available in PMC: 2020 Nov 23.
Published in final edited form as: Biochem Pharmacol. 2019 Aug 14;169:113604. doi: 10.1016/j.bcp.2019.08.006

Table 2:

Gradient elution program with 100 μl injection volume of the urine samples

Step Time (min) Flow (ml/min) Mobile Phase A Mobile Phase B Curve
0 2.0 1.20 100 0 0.0
1 2.0 1.20 85 15 6.0
2 25.0 1.20 0 100 2.0
3 7.0 1.20 100 0 2.0