Figure 4.
GGPPS deletion significantly impaired neonatal aorta smooth muscle by GGPP depletion. A, primary aorta smooth muscle cells isolated from GGPPSSMKO and CTR mice at the age of 14 days were incubated with or without 10 μm GGPP (n = 5). Scale bars, 100 μm. B, cell proliferation of aorta smooth muscle cells from GGPPSSMKO and CTR mice with or without 10 μm GGPP (n = 3) was tested by CCK-8. C, aorta smooth muscle cells were stained with DAPI and smooth muscle marker, SMA and SMMHC (n = 3). Scale bars, 50 μm. Data of B are presented as mean ± S.E. (error bars) of biologically independent samples with two-way ANOVA followed by Bonferroni's test. ns, p > 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001.