Figure 8.

Comparison of the two in vitro and two in vivo activities for each of the OST complexes containing a mutated Stt3 subunit. The in vitro data were obtained with the purified OST[Ost3, PA-Stt3(X)] proteins, whereas the in vivo data were obtained with the yeast strains stt3Δ png1Δ ams1Δ-pSTT3(X), where X denotes a mutation. These data are redisplayed from Figs. 4 (A and B) and 5 (A and C) for a detailed comparison. Welch's t test was performed between the WT and each mutant. *, p < 0.05. The growth phenotype and the time required to reach OD₆₀₀ 10 from 0.2 in the YPD medium at 30 °C are shown.