Figure 3.

Pif1 disrupts nucleosomes to allow through-replication. A, schematic of primer extension assays performed on DNA substrates with a nucleosome assembled on a 601 Widom sequence placed 20 bp from a nick. B, representative sequencing gels of primer extension assays performed using Pol δ^DV with or without a nucleosome, RPA, and/or Pif1 and monitored over time (1, 2, 5, 10, 20, and 30 min). C, lane plots of the band intensity of the 30-min time point for primer extension assays on nucleosome-bound substrates in B.