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. 2020 Aug 18;295(47):15853–15869. doi: 10.1074/jbc.RA120.014542

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© 2020 Stapane et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — Western blotting analysis of (A) EDIL3 and (B) MFGE8 in UF: unfractioned UF (complete), depleted UF (ØEV), EV fraction of UF (EV fraction), and SEP. Proteins (15 µg) were subjected to 12.5% SDS-PAGE electrophoresis and blotted for analysis. The membranes were probed with (A) rabbit polyclonal anti-EDIL3 (1:1,000, SAB2105802, Sigma-Aldrich, Saint-Quentin Fallavier, France) and (B) rabbit synthetic anti-MFGE8 peptide (1:1,000, rabbit polyclonal; ProteoGenix, Schiltigheim). White arrowheads indicate the immunoreactive bands.