. 2020 Aug 18;295(47):15853–15869. doi: 10.1074/jbc.RA120.014542

Table 1.

Normalized vesicular mRNA levels in the four oviduct regions and other tissues

Messenger RNA level results are reported as mean ± S.D. Messenger RNA level was assessed during the active growth phase of eggshell mineralization at 10 h p.o. except for B at 18 h p.o. Following reverse transcription, the mRNA level was quantified using primers designed with Primer-blast tool of NCBI (RRID:SCR_003095) and validated for the Biomark microfluidic system (BMK-M-96.96; Fluidigm). Relative quantification was normalized with eight housekeeping genes using GenNorm software. Superscript letters “a” to “e” correspond to significance results of the ANOVA-Tukey tests with “a” the letter corresponding to the highest level of mRNA for each gene. Tibial bone, B; duodenum, D; kidney, K; liver, L; magnum, Ma; white isthmus, WI; red isthmus, RI; uterus, Ut. The gene accession numbers and primer sequences are compiled in Table S3.

Function	Tissues					Oviduct segments
Function	Gene	B	D	K	L	Ma	WI	RI	Ut	ANOVA p value
Calcium channel	Anxa1	0.205 ± 0.200^cd	0.002 ± 0.001^d	0.009 ± 0.004^d	0.001 ± 0.001^d	0.194 ± 0.066 ^cd	2.190 ± 0.749^b	3.338 ± 0.768^a	0.891 ± 0.320^c	<0.001
	Anxa2	0.307 ± 0.240^cd	0.521 ± 0.180 ^cd	0.006 ± 0.009^d	0.001 ± 0.002^d	0.543 ± 0.340 ^cd	1.208 ± 0.805^bc	2.884 ± 1.247^a	1.904 ± 0.459^ab	<0.001
	Anxa5	0.431 ± 0.359	0.401 ± 0.289	0.189 ± 0.173	1.462 ± 0.828	2.001 ± 2.920	0.560 ± 0.298	1.405 ± 1.044	0.298 ± 0.193	0.068
	Anxa6	2.116 ± 1.522^ab	0.446 ± 0.282^b	0.313 ± 0.163^b	1.473 ± 0.558^ab	1.328 ± 1.140^ab	0.478 ± 0.375^ab	0.347 ± 0.147^b	0.543 ± 0.437^ab	0.008
	Anxa7	0.206 ± 0.081^cd	1.436 ± 0.306^b	7.074 ± 1.170^a	0.191 ± 0.059^d	0.623 ± 0.330^bcd	0.676 ± 0.410^bcd	1.122 ± 0.284^bc	1.450 ± 0.526^b	<0.001
	Anxa8	0.026 ± 0.029^d	0.001 ± 0.001^d	0.001 ± 0.001^d	0.001 ± 0.001^d	1.669 ± 0.922^bc	2.513 ± 1.205^ab	3.958 ± 1.720^a	0.979 ± 0.344 ^cd	<0.001
	Anxa11	0.214 ± 0.044	0.562 ± 0.152	0.644 ± 0.293	0.916 ± 0.939	3.760 ± 6.150	2.180 ± 3.080	1.615 ± 1.436	1.008 ± 0.647	0.236
Bicarbonate supplier/transporter	Ca²⁺	21.440 ± 10.270^a	2.585 ± 1.127^b	1.633 ± 0.834^b	0.512 ± 0.157^b	0.763 ± 0.196^b	1.149 ± 0.297^b	0.561 ± 0.171^b	1.131 ± 0.394^b	<0.001
	Slc4a7	0.115 ± 0.039^cd	1.905 ± 0.665^a	0.069 ± 0.008 ^cd	0.012 ± 0.007^d	0.454 ± 0.149 ^cd	0.551 ± 0.287^c	1.078 ± 0.262^b	0.529 ± 0.120^c	<0.001
Membrane organizer	Cd82	0.634 ± 0.345^cd	0.769 ± 0.310^bcd	0.367 ± 0.241 ^cd	0.162 ± 0.141^d	1.598 ± 0.970^ab	0.943 ± 0.260^abcd	1.270 ± 0.403^abc	1.865 ± 0.834^a	<0.001
	Cd9	0.085 ± 0.100^b	0.258 ± 0.129^b	0.403 ± 0.168^b	0.053 ± 0.068^b	1.751 ± 1.255^b	12.900 ± 4.98^a	1.780 ± 0.323^b	1.617 ± 1.106^b	<0.001
Chaperone	Hsp90b1	2.427 ± 1.776^a	0.941 ± 0.672^a	3.230 ± 3.500^a	5.250 ± 3.920^a	4.560 ± 4.650^a	1.649 ± 0.760^a	1.627 ± 0.659^a	2.992 ± 0.840^a	0.039
	Hspa8	0.562 ± 0.125^d	0.840 ± 0.203^bcd	1.180 ± 0.334^b	0.404 ± 0.151^d	0.973 ± 0.249^bc	0.919 ± 0.287^bc	1.709 ± 0.399^a	1.084 ± 0.205^b	<0.001
Adhesion molecule	Itgb1	1.925 ± 0.947^a	0.345 ± 0.084^bc	0.671 ± 0.491^abc	0.133 ± 0.064^c	1.827 ± 0.961^a	1.536 ± 0.826^ab	1.783 ± 1.054^a	1.396 ± 0.504^abc	<0.001
Adhesion molecule	Edil3	0.019 ± 0.017^b	0.000 ± 0.000^b	0.001 ± 0.003^b	0.000 ± 0.000^b	0.000 ± 0.000^b	0.825 ± 0.303^a	1.101 ± 0.737^a	0.737 ± 0.464^a	<0.001
	Mfg8	0.514 ± 0.380^c	0.114 ± 0.078^c	0.856 ± 0.208^c	4.373 ± 1.223^a	0.029 ± 0.063^c	0.803 ± 0.399^c	2.139 ± 1.194^b	0.381 ± 0.385^c	<0.001
Intracellular trafficking	Rab11a	0.399 ± 0.125^d	2.575 ± 0.600^a	0.993 ± 0.110^b	0.506 ± 0.079 ^cd	1.110 ± 0.201^b	0.703 ± 0.130^bcd	0.952 ± 0.076^bc	1.116 ± 0.226^b	<0.001
	Rab27a	0.381 ± 0.168^c	0.329 ± 0.197^c	0.585 ± 0.283^c	0.185 ± 0.144^c	2.306 ± 1.363^a	1.778 ± 0.517^ab	1.090 ± 0.485^bc	1.742 ± 0.634^ab	<0.001
	Rab5a	0.525 ± 0.353^bc	0.779 ± 0.251^bc	0.784 ± 0.324^bc	0.780 ± 0.422^bc	1.701 ± 0.881^a	1.337 ± 0.500^abc	1.294 ± 0.300^abc	1.649 ± 0.596^ab	<0.001
	Rab7a	1.879 ± 0.849	1.399 ± 0.781	0.991 ± 0.381	1.016 ± 0.500	1.375 ± 0.523	1.258 ± 0.335	1.353 ± 0.377	1.509 ± 0.354	0.297
	Ralb	0.987 ± 0.257^ab	1.034 ± 0.658^ab	1.350 ± 0.587^a	1.191 ± 0.484^ab	0.679 ± 0.939^ab	0.615 ± 0.371^ab	0.644 ± 0.438^ab	0.349 ± 0.203^b	0.039
	Vcp	1.116 ± 0.495^ab	0.414 ± 0.136^b	0.707 ± 0.259^b	2.129 ± 0.802^a	1.379 ± 1.746^ab	1.071 ± 0.350^ab	1.229 ± 0.469^ab	1.334 ± 0.519^ab	0.027
	Arf6	0.597 ± 0.141^bc	1.688 ± 0.490^a	0.770 ± 0.201^bc	0.702 ± 0.360^bc	0.647 ± 0.255^bc	0.453 ± 0.194^c	0.949 ± 0.305^bc	1.206 ± 0.370^ab	<0.001
	Sdcbp	0.581 ± 0.203^d	0.920 ± 0.225^cd	2.398 ± 0.326^ab	0.546 ± 0.210^d	2.585 ± 1.130^a	2.506 ± 0.629^ab	2.013 ± 0.386^ab	1.567 ± 0.422^bc	<0.001
EV biogenesis	Tsg101	0.134 ± 0.018^e	0.510 ± 0.136^cd	0.764 ± 0.180^bc	0.386 ± 0.056^de	1.206 ± 0.385^a	0.900 ± 0.224^ab	1.088 ± 0.134^ab	1.192 ± 0.184^a	<0.001
EV biogenesis	Vps26a	0.447 ± 0.271^de	0.831 ± 0.186^cde	1.078 ± 0.147^bcd	0.466 ± 0.183^de	1.203 ± 0.419^abcd	1.754 ± 0.338^ab	1.937 ± 0.657^a	1.417 ± 0.771^abc	<0.001
	Pdcd6ip	0.213 ± 0.047^e	1.150 ± 0.225^bc	0.727 ± 0.156^cde	0.493 ± 0.109^de	0.777 ± 0.332^cd	0.842 ± 0.303 ^cd	1.454 ± 0.301^b	2.030 ± 0.545^a	<0.001
EV release	Vamp3	0.199 ± 0.060^d	0.575 ± 0.088^bc	0.365 ± 0.124 ^cd	0.156 ± 0.080^d	0.682 ± 0.190^b	0.751 ± 0.122^b	1.117 ± 0.253^a	0.749 ± 0.134^b	<0.001
	Vamp7	0.439 ± 0.224^de	0.590 ± 0.281^cde	1.527 ± 0.542^ab	1.088 ± 0.258^abcd	0.911 ± 0.258^bcde	1.198 ± 0.242^abc	1.407 ± 0.552^ab	1.704 ± 0.373^a	<0.001
	Vps4b	0.014 ± 0.010^b	0.041 ± 0.020^ab	0.019 ± 0.019^b	0.009 ± 0.006^b	0.052 ± 0.029^ab	0.028 ± 0.022^b	0.029 ± 0.017^b	0.090 ± 0.069^a	<0.001
	Ap1g1	0.613 ± 0.163^ab	0.649 ± 0.154^ab	0.509 ± 0.097^ab	0.334 ± 0.185^b	0.824 ± 0.539^ab	0.726 ± 0.404^ab	1.063 ± 0.260^ab	1.199 ± 0.465^ab	0.030
Signaling protein	Ywhah	0.338 ± 0.089^abcd	0.517 ± 0.310^abcd	0.171 ± 0.104^cd	0.111 ± 0.098^d	0.970 ± 0.339^ab	1.071 ± 0.683^ab	1.089 ± 0.562^a	0.872 ± 0.568^abc	<0.001
	Ywhaz	0.485 ± 0.200^cd	0.501 ± 0.152^cd	0.507 ± 0.131^cd	0.136 ± 0.050^d	0.660 ± 0.266^c	0.786 ± 0.159^bc	1.192 ± 0.291^b	2.121 ± 0.394^a	<0.001