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. 2020 Oct 13;19(11):e13166. doi: 10.1111/acel.13166

Figure 5.

Figure 5

Increased mtDNA point mutations fail to disrupt insulin sensitivity or promote adiposity in aged mice. Mitochondrial DNA (a) point mutation frequency and (b) DNA copy number in quadriceps muscle from PolG+/mut relative to normalized Control. (c) Weekly body weight in aging mice. (d) Wet tissue weights relative to body weight of PolG+/mut versus Control. (e–h) Muscle strength and endurance. (i–k) Plasma triglyceride, glucose, and lactate concentrations. (l) Insulin tolerance test with area under the curve (AUC) insert. (m) Hepatic lipid levels expressed as milligram of lipid/gram of liver (TG, triglyceride; TC, total cholesterol; UC, unesterified cholesterol; PC, phosphatidylcholine; CE, cholesterol ester). (n) Muscle expression of genes encoded by the mitochondrial genome. (o–p) Muscle oxygen consumption rates from complex I and complex IV expressed as picomoles of oxygen/minute/microgram of quadriceps protein using NADH or succinate + rotenone as substrates. (q) Expression of proteins/phosphoproteins in muscle from PolG+/mut relative to Control. Values are expressed as means ± SEM. Mean differences were detected by Student's t test. *p < .05, significantly different from Control. N = 8–15/group