Figure 6.

Increased mtDNA point mutations alter the mitochondrial proteome of muscle from aged mice. (a) Representative immunoblot verifying cytosolic (Cyto), nuclear (Nuc), and mitochondrial (Mito) fractions of control muscle. (b) Selected mitochondrial protein, fission factor MFF, to corroborate findings from a mitochondrial proteomic screen compared with immunoblotting. (c) Unbiased hierarchical clustering of mice indicating genotype and sample #. (d) Volcano plot of proteins identified in the muscle mitochondrial proteomic screen expressed as fold change for 12‐month‐old PolG^+/mut relative to Control (dotted line indicates significance threshold, and blue dots indicate proteins significantly altered in PolG^+/mut relative to control). (e) Pathway analysis of significantly altered proteins in muscle from aged PolG^+/mut relative to Control (dotted line indicates significance threshold). (f) Functional annotation clustering of significantly altered proteins identified in mitochondrial proteomic screen expressed as an enrichment score. (g) Transcription factor association of significantly altered proteins expressed as significance of association from TRRUST analysis (dotted line indicates significance threshold). * p < .05 significantly different from Control. N = 5/group