Figure 3.

Ovarian stromal Has3 and Hyal1 undergo age‐dependent transcriptional changes independent of estrous cyclicity. Relative gene expression levels of (a) hyaluronan synthases and (b) hyaluronidases in ovarian stroma from reproductively young and old mice. The data are shown as a fold change expression over Has1 (a) or Hyal1 (b) in reproductively young mice. (c) A representative plot of a mouse exhibiting normal estrous cyclicity over a 15‐day period. (d) Graph showing the relative expression of Has3 and Hyal1 in reproductively young and old mice with normal estrous cycles. (e) A representative plot of a mouse exhibiting abnormal estrous cyclicity over a 15‐day period. (f) Relative expression of Has3 and Hyal1 in reproductively young and old mice with abnormal estrous cycles. (c and e) 0‐3 represent the different cycle stages: diestrus (0), proestrus (1), estrus (2), and metestrus (3). N = 30 stromal samples from reproductively young mice; N = 29 stromal samples from reproductively old mice. Asterisks indicate significant differences (p < 0.05)