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. 2020 Nov 6;7:586413. doi: 10.3389/fmolb.2020.586413

FIGURE 2.

FIGURE 2

The 3′-end-sharing mRNA species are the intermediate products of the RNase E-mediated decay of galETKM. (A) Northern blot of the gal mRNA in GW20 (ams1ts) cells grown at either the permissive temperature of 30°C (lane 1) or the non-permissive temperature of 44°C (lane 2). The blot was probed with the M probe complementary to the 3′ half of the galM gene of the gal operon. galETKM and galM1 are the primary transcripts, and galTKM, galKM, and galM are the decay intermediates. (B) 5′RACE of gal mRNA prepared from GW20 (ams1ts) cells as in (A). The lanes show the 5′ ends of RNA at the permissive and the non-permissive temperatures found near the beginning of either the galT gene (lanes 1 and 2) or the galK gene (lanes 3 and 4) or the galM gene (lanes 5 and 6). DNA sequencing ladders used as molecular weight markers are in lanes marked G and A. An exception was the 5′ end at 1,061, which was unchanged at the two temperatures, indicating that this 5′ end was not generated by RNase E.