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. 2021 Jan 1;11(1):64–78. doi: 10.7150/thno.46124

Figure 3.

Figure 3

In vitro evaluation of nanocatalytic performances of GE@EVs and intracellular ROS production efficiency. (A) Schematic image of nanocatalytic procedures of GE@EVs in mildly acidic condition. (B) ESR spectra of GE@EVs (10 µg/mL) with or without (blank) the addition 10 mM glucose. (C-H) Michaelis-Menten steady-state kinetics of GE@EVs. (C, F) Time-course absorbance of GE@EVs upon the addition of varied concentrations of β-D-glucose (10, 2, 1 and 0.5 mM) and H2O2 (50, 25, 12.5, and 5 mM). (D, E) Michaelis-Menten kinetics and Lineweaver-Burk plotting of GE@EVs with addition of β-D-glucose. (G, H) Michaelis-Menten kinetics and Lineweaver-Burk plotting of GE@EVs with addition of H2O2. (I) CLSM images of intracellular ROS production. (Scale bar = 50 µm).