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. 2021 Jan 1;11(1):64–78. doi: 10.7150/thno.46124

Figure 4.

Figure 4

In vitro cytotoxicity profiles and nanocatalytic therapy against HCC Huh 7 cells. (A) Schematic illustration of nanocatalytic therapy against Huh 7 cells. (B) Cytotoxicity of ESIONs-PEG, ESIONs-RGD and ESIONs-RGD@EVs after the incubation with Huh 7 cell lines for 24 h. (C) Relative viabilities of Huh 7 cells at different concentrations of GE@EVs in mildly acidic and neutral solution. (D) L-ascorbic acid-assisted cell rescue profiles of Huh 7 cells cytotoxicity induced by 100 µg/mL of GE@EVs. (E) Red fluorescent quantitation and (F) CLSM images of Huh 7 cells stained by calcein AM (green) and PI (red) incubated for 2 and 4 h after different treatments (Scale bar = 40 µm). (G) Invasion rate results of transwell images after different incubations. (H) Transwell images and cellular invasion profiles of Huh 7 cells after different incubations (Scale bar = 100 µm). (I) Bio-TEM images of intracellular organelles after different treatments. The white arrow points out the normal cell shape, and white dotted line circle represents the swollen and deformed mitochondria and golgi bodies (Scale bar = 1 µm). *P < 0.05 and **P < 0.01.