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. 2021 Jan 1;11(1):410–425. doi: 10.7150/thno.51046

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Immunofluorescent labeling of DAPI (blue), Ki67 (green) and CD11b (red) in the ischemic region, shown as three channels. The data show proliferative (Ki67⁺), microglia/macrophages (CD11b⁺) at day 7 after MCAO in DPCPX (upper row), vehicle (middle row), and ENBA-treated rats (lower row). Proliferative CD11b-reactive microglia/macrophages decrease after MCAO in ENBA-treated rats (see merged images) (D). The number of CD11b-reactive microglia/macrophages showing positive Ki67 was evaluated at day 7 after daily treatment with DPCPX (n = 5), Vehicle (n = 8) and ENBA (n = 6) (E). *p < 0.05 compared with MCAO+ENBA. Scale bars, 10 µm. Values are presented as scatter dot blot (mean ± SD).