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. 2021 Jan 1;11(1):330–345. doi: 10.7150/thno.51004

Figure 2.

Figure 2

dSTORM images and distance analysis revealed postsynaptic localization of synaptic TDP-43. (A) Representative wide-field (WF) and dSTORM images of the presynaptic marker, Bassoon, and TDP-43 are shown. Arrowheads indicate the nearby colocalization of Bassoon and TDP-43 in magnified dSTORM imaging (white rectangle). (B) Representative wide-field and dSTORM images of TDP-43 and the postsynaptic marker PSD-95 are shown. Arrowheads indicate near-by colocalization of PSD-95 and TDP-43 in magnified dSTORM imaging (white rectangle). Scale bars: 10 µm and 5 µm (magnified dSTORM images). (C) Representative dSTORM images show the synaptic pairs between PSD-95, Bassoon and TDP-43 (left panels). Scale bar: 400 nm. Histograms showing the distribution of measured distance between PSD-95 and Bassoon, TDP-43 and Bassoon, PSD-95 and TDP-43 (right panels). The data represent the distance of n ≥ 25 synaptic pairs in n ≥ 3 biological repeats.