Table IV.
Methylated DNA as biomarker for detection of gastric cancer.
| Diagnostic value, % (n) | ||||||
|---|---|---|---|---|---|---|
| cfDNA | Biomarker candidate | Sample type | Method | Sensitivity | Specificity | (Refs.) |
| MethyDNA | P16 | Serum | MSP | 26 (6/23) | 100 (16/16) | (64) |
| Serum | MSP | 18 (20/109) | 100 (10/10) | (65) | ||
| Blood | MSP | 72.6 (77/106) | 94.4 (17/18) | (93) | ||
| Serum | MSP | 51.9 (28/54) | 100 (30/30) | (98) | ||
| Plasma | MSP/HPLC | 14.3 (12/84) | 100 (15/15) | (110) | ||
| Serum | MSP | 26.9 (14/52) | 100 (29/29) | (67) | ||
| Serum | MSP | 22 (9/41) | 100 (10/10) | (111) | ||
| P16/E-Cadherin | Serum | MSP | 37 (40/109) | 100 (10/10) | (65) | |
| E-Cadherin | Serum | MSP | 24 (26/109) | 100 (10/10) | ||
| Serum | MSP | 22 (9/41) | 100 (10/10) | (111) | ||
| p16/E-cadherin/RARbeta | Serum | MSP | 44 (18/41) | 100 (10/10) | ||
| RUNX3 | Tissues and cell lines | MSP | 64 (48/75) | (69) | ||
| Serum | rt-MSP | 70.8 (143/202) | 99.8 (848/850) | (71) | ||
| Serum | qMSP | 95.5 | 62.5 | (70) | ||
| Plasma | MSP | 42.7 (56/131) | 100 (34/34) | (72) | ||
| Plasma ZIC1, HOXD10 and RUNX3 | Plasma | MSP | 91.6 | 50.0 | ||
| Zic1 | Plasma | MSP | 69.5 (91/131) | 100 (34/34) | ||
| RASSF1A | Serum | MSP | 34 (16/47) | 100 (30/30) | (74) | |
| Serum | MSP | 68.5 (50/73) | 100 (20/20) | (75) | ||
| Plasma | MSP | 83.2 (84/101) | 94.55 (191/202) | (76) | ||
| PCDH10 | Plasma | MSP | 94.1 (95/101) | 97.03 (142/202) | ||
| Reprimo | Plasma | MSP | 95.3 (41/43) | 90.3 (28/31) | (79) | |
| Plasma | MSP | 86.3 (44/51) | 97.6 (48/49) | (81) | ||
| Plasma | MSP | 62 (31/50) | 100 (30/30) | (80) | ||
| hMLH1 | Plasma | MSP | 48 (24/50) | 96.7 (29/30) | ||
P16, Cyclin-dependent kinase inhibitor 2A, multiple tumor suppressor 1; E-Cadherin, epithelial cadherin; RARbeta, retinoic acid receptor beta; RUNX3, Runt-related transcription factor 3; ZIC1, zinc finger of the cerebellum 1; RASSF1A, Ras association domain-containing protein 1; PCDH10, protocadherin-10 gene; hMLH1, MutL homolog 1; MSP, methylation-specific polymerase chain reaction; HPLC, high performance liquid chromatography.