Figure 1.

Silencing TLR4 in U-87MG cells using shRNA plasmids. (A) Transfection efficiency was evaluated using flow cytometry. The percentage of cells expressing green fluorescence measured using flow cytometry represents the transfection efficiency. ^#P<0.01 vs. TLR4-sh2. The letters “C” and “D” represent the gate of flow cytometry. (B) Expression levels of TLR4 mRNA in transfected cells using fluorescence reverse transcription-quantitative PCR. *P<0.05 vs. blank and negative control. n=3. TLR4, toll-like receptor 4; sh, short hairpin RNA.