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. 2020 Nov 6;8:600641. doi: 10.3389/fcell.2020.600641

FIGURE 3.

FIGURE 3

miR-129-5p inhibited downstream transcript factors of Wnt/β-catenin pathway. (A,B) Correlation analysis between miR-129-5p and Tcf7 or Lef1 mRNA levels in femur tissues from C57BL/6 mice, respectively, as detected by reverse transcriptase-polymerase chain reaction (RT-PCR). (C,D) Tcf7 and Lef1 expression levels of MC3T3-E1 cells treated with mimic-129-5p, as detected by RT-PCR (mean ± SD, ∗∗∗P < 0.001). NC, mimic-NC; mimic-129, mimic-129-5p. (E) TCF7/LEF1 activities of MC3T3-E1 cells treated with mimic-129-5p, as detected by luciferase reporter assay (mean ± SD, ∗∗∗P < 0.001). (F,G) Tcf7 and Lef1 expression levels of MC3T3-E1 cells treated with inhibitor-129-5p, as detected by RT-PCR (mean ± SD, ∗∗P < 0.01, ∗∗∗P < 0.001). NC, inhibitor-NC; inhibit-129, inhibitor-129-5p. (H) TCF7/LEF1 activities of MC3T3-E1 cells treated with inhibitor-129-5p, as detected by luciferase reporter assay (mean ± SD, ∗∗P < 0.01).