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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Neuroscience. 2020 Jan 23;443:188–205. doi: 10.1016/j.neuroscience.2020.01.020

Table 2:

Antibodies used for single-labeling immunofluorescence histochemistry

Primary antibody Dilution Vendor Secondary antibody Dilution Vendor
Rabbit anti-CCL2 1:200 Biorbyt, CA Bio-Horse anti-Rabbit IgG + TSA Fluorescin 1:100 Vector, CA
Rabbit anti-BLBP 1:200 Abcam, MA Cy3-Donkey anti-Rabbit IgG 1:100 Jackson ImmunoResearch Laboratories Inc, PA
Rabbit anti-MCH 1:1000 Phoenix Cy3-Donkey anti-Rabbit IgG 1:100 Jackson ImmunoResearch
Pharmaceuticals, CA Laboratories. Inc, PA
Rabbit anti-Iba-1 1:1000 Wako Chemicals USA, VA Cy3-Donkey anti-Rabbit IgG 1:100 Jackson ImmunoResearch
Laboratories. Inc, PA
Mouse anti-GFAP 1:50 EMD Millipore, MA Cy3-Donkey anti-Mouse IgG 1:100 Jackson ImmunoResearch
Laboratories. Inc, PA

Abbreviations: CCL2, C-C motif ligand 2; BLBP, brain lipid-binding protein, marker for radial glia; MCH, melanin-concentrating hormone; Iba-1, marker for microglia; GFAP, marker for astrocytes.