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. Author manuscript; available in PMC: 2020 Nov 23.
Published in final edited form as: Science. 2020 Apr 3;368(6486):85–89. doi: 10.1126/science.aaw9872

Fig. 1. Pancreatic cancer cells require exogenous cystine to avert ferroptosis.

Fig. 1.

(A and B) Viability of human PDAC lines after 24 hours culture in varying concentrations of cystine (A) or IKE (B), alone or in combination with 100 μM Trolox. Student’s t-test comparing maximal cytotoxicity ± Trolox. (C) Viability of PANC-1 cells cultured for 24 hours in cystine-free media or treated with 10 μM IKE, alone or in combination with 100 μM Trolox (Tro), 500 nM ferrostatin-1 (Fer1), 100 μM deferoxamine (DFO), 1mM N-acetyl cysteine (NAC), 50 μM ZVAD-FMK, 1 nM Bafilomycin A1 (BA1) or 10 μM Necrostatin-1s (Nec1s). Tukey test. (D) Flow cytometry of C11-BODIPY fluorescence in PANC-1, AsPC-1, BxPC-3, and S-2013 cells after 6–8 hours of treatment with conditions from panel C. Tukey test. All data are means ±SEM of three independent experiments. * p < 0.05, x = no significant difference.