Fig. 4.

In situ hybridization using DIG-labeled riboprobes for Ptep-soA in late embryos of the spider Parasteatoda tepidariorum. All embryos in frontal view. a–d: bright field images. A’–D’: Same embryos, in Hoechst staining. a: Sense probe of a stage 14.2 embryo (no signal). b: Antisense probe on a wild type stage 14.1 embryo. c: Antisense probe on a wild type stage 14.2 embryo. d: Antisense probe on a stage 14.2 embryo from the Ptep-soA dsRNA-injected treatment. soA: sine oculis A. White arrowhead: median eye; Black arrow: anterior lateral eye; White arrow: median lateral eye; Double white arrow: Posterior lateral eye. Dotted arrowhead/arrow indicate asymmetrical expression and eye defect. Sample sizes are indicated above each treatment