Fig. 4.

KCNA2-AS binds to pSTAT3 and regulates its activation. Primary rat spinal astrocytes were transfected with or without si-KCNA2-AS, and treated with GSNO for 24 h. The control group was untreated cells. a Immunofluorescence staining was performed with the pSTAT3 antibody. pSTAT3, red staining. b The nuclear or total STAT3 and pSTAT3 protein levels were detected by Western Blot. **P < 0.01 vs. control group; ^#P < 0.05 vs. GSNO group. c RNA fluorescence in situ hybridization was performed. KCNA2-AS, red staining; DAPI/nucleus, blue staining. The nuclear or cytoplasm of KCNA2-AS levels were detected by qRT-PCR. d–e Primary rat spinal astrocytes were treated with GSNO for 24 h. The interaction between KCNA2-AS and pSTAT3 was assessed using RNA pull-down assay (d) and RIP assay (e). **P < 0.01 vs. Anti-IgG group