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. 2020 Nov 23;19:164. doi: 10.1186/s12943-020-01272-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Circ-ERBIN accelerates the growth and metastasis of CRC by activating HIF-1α signaling through the circ-ERBIN/miR-125a-5p/miR-138-5p/ 4EBP-1 pathway. a Transwell assays for HCT116 cells that stably overexpressed circ-ERBIN or co-transfected with miR-125a-5p or miR-138-5p mimics after 2 days. b MiR-125a-5p or miR-138-5p inhibitor were transfected alone or together into HCT116 sh-circ-ERBIN #1 stable cell lines for 2 days followed by the analysis of cell migration using transwell assays. Cells were stained by Giemsa’s staining and visualized under a phase-contrast microscope. c-e HCT116 stably expressed circ-ERBIN or pLCDH vector were injected into BALB/C nude mice (n = 5 for each group). AgomiRs were injected into tumors as represented. The tumors were excised and photographed, and the size and weight of tumors were presented. f Representative immunohistochemical stainings of 4EBP-1 and HIF-1α in subcutaneous tumors with agomiRs injection as described. Scale bars, 100 μm. g A schematic model displaying the role of circ-ERBIN/miR-125a-5p/miR-138-5p/ 4EBP-1/HIF-1α axis in CRC metastasis.