Fig. 2.

Block of T-cell development in knock-in mice expressing the phosphomimetic Lck^Y192E mutant. a Total numbers of thymocytes obtained from Lck^wt and Lck^Y192E knock-in mice. b Thymic subpopulations were identified upon staining with CD4 and CD8 antibodies by flow cytometry. c Percentages of the thymocyte subpopulations of several experiments are shown. d Numbers of total peripheral T cells and of peripheral CD4⁺ and CD8⁺ T cell subsets. Each dot represents one mouse. Mean values of all characterized mice + SEM are represented. Statistical analyses were performed using an unpaired Student’s t test, ****p < 0.0001; ***p < 0.001