Fig 2.

SRSF1 is upregulated by BCR-ABL1 and extrinsic cytokine signaling. a. Western blots were performed on cell lysates from K562 and KYO-1 cells treated with increasing doses of IM for 48 h, (n=3). b. Western blots from isogenic cell lines, Ba/F3 parental and Ba/F3 p210, cultured in 10 ng/ml recombinant murine IL-3 and/or 1.5 μM IM for 24 h, (n=3). c. Western blots from CP CML CD34⁺ cells from two different patients treated with 1 μM imatinib (IM) for 16 h and supplemented with either ‘low’ or ‘high’ cytokines. d. Colony forming assays were performed using cells from CP CML CD34⁺ cells from 3 individual patients. CD34⁺ cells were cultured in either low or high cytokine levels and treated with 1 μM IM for 96 h. Following treatment, cells were plated in methylcellulose, and colony forming units (CFU) scored 14-18 days later. Colony numbers are represented relative to the low cytokine control in DMSO. Samples under low and high cytokine conditions from the same individual are connected by lines, p-values are determined by Student’s t-test.