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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Leukemia. 2020 Feb 12;34(7):1787–1798. doi: 10.1038/s41375-020-0732-1

Fig 6.

Fig 6

Targeting PKC signaling rescues SRSF1-mediated impaired imatinib responses a. Levels of PRC and PLC genes determined by RNA-sequencing of K562 cells overexpressing SRSF1. Transcript levels have been determined by normalizing Log2 counts. b. CFU from SRSF1-overexpressing K562 cells transfected with non-targeting control or siPRCKH/ PLCG2/ PLCH1 (n=3), treated with 0.5μM IM for 48h. IM treatment was started 48 h after transfection with the respective siRNA pools. Error bars represent ±SEM. p-values are determined by 2 way ANOVA, *p < 0.05; **p < 0.01; ***p < 0.001 All CFU numbers are represented relative to their respective controls and were scored 10-14 days after plating. c. Western blots from CD34+ CP CML progenitor cells following SRSF1 depletion with either a non-targeting siRNA control or siSRSF1. Cells were cultured in low cytokines and harvested for protein lysates 48h post-transfection.