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. 2020 Nov 23;39:251. doi: 10.1186/s13046-020-01774-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — WNT4 promote angiogenesis in colorectal cancer. a and b The expression of CD31 (red) in different groups: LoVo-Scramble or LoVo-shWNT4 (a), SW480-WNT4-vector or SW480-WNT4-HA. Scale bar, 50 μm. c The ability of tube formation was significantly improved when HUVECs were treated with conditioned media (CM) from SW480 cells transfected with WNT4-HA than that from SW480 transfected WNT4-vector, and it could be blocked by the β-catenin/TCF inhibitor ICG-001 (10 μM). Scale bar, 100 μm. d TOP/FOP-Flash reporter was used to detect whether the WNT pathway was activated. e and f The levels of ANG2 were detected by RT-qPCR and ELISA in HUVECs that underwent different treatments. g and h The mRNA level of ANG2 in cells and CM of HUVECs transfected with negative control shRNA (Scramble) and sh-ANG2 were detected by RT-qPCR. i The ability of tube formation promoted by high WNT4 expression was significantly inhibited due to low ANG2 expression. Scale bar, 100 μm. **P < 0.01