Fig. 4.

Regulation of CrvA expression occurs at the posttranscriptional level. (A) Normalized luciferase reporter activity (relative light units [RLUs]) of PcrvA–luciferase transcriptional fusion as a function of cell density (OD₆₀₀) under high (QrgB, dark) and unaltered (QrgB*, light) c-di-GMP conditions in populations grown to early stationary phase supplemented with 100 µm IPTG. Dots represent pooled data from four biological replicates. (B) qRT-PCR analysis of relative crvA transcript levels between high (QrgB) and unaltered (QrgB*) c-di-GMP conditions. Each dot represents one independent replicate. (C) Western blot (Left, representative plot) and quantitative intensity analysis (Right, all blots) of CrvA-HIS under high (QrgB, dark) or unaltered (QrgB*, light) c-di-GMP conditions in the parent (Left) and ∆vpsT (Right) strains grown to early stationary phase supplemented with 100 µM IPTG. Shapes represent independent replicates and y axis is log10. (Inset) Estimated effects of QrgB and VpsT expression on CrvA accumulation. (dot = mean, thick line = 90% CI, thin line = 98% CI).