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. 2020 Nov 19;12:11883–11895. doi: 10.2147/CMAR.S267927

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© 2020 Huang et al.

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CirSLC19A1 directly targeted miR-326, a down-regulated gene in PCa tissue. (A) The abundances of circSLC19A1 and SLC19A1 in the cytoplasm and nucleus of DU145 or PC3 cells were assessed by qRT-PCR, with GAPDH and U6 serving as reference genes. (B) RegRNA2.0 predicted the mutual binding sites between circSLC19A1 and miR-326. (C) Dual-luciferase reporter assay confirmed the interaction between circSLC19A1 and miR-326. (D) miR-326 expression in DU145 and PC3 cells transfected with or without sicircSLC19A1 was assessed by qRT-PCR, with U6 serving as a reference gene. (E) miR-326 expression in PCa tissues and the adjacent normal tissue tissues was assessed by qRT-PCR, with U6 serving as a reference gene. (F) Spearman correlation analysis reflected the correlation between circSLC19A1 and miR-326. **P<0.01 and ***P<0.001 vs IC; ^{^^^}P<0.001 vs siNC; ^###P<0.001 vs ANT.

Abbreviations: PCa, prostate cancer; sicircSLC19A1, siRNA-specific target circSLC19A1; siNC, siRNA-negative control; WT, wild type; MUT, mutant type; ANT, adjacent normal tissue; qRT-PCR, quantitative reverse-transcription polymerase chain reaction.