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. 2020 Nov 19;12:11883–11895. doi: 10.2147/CMAR.S267927

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© 2020 Huang et al.

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MAPK1, a target gene of miR-326, was up-regulated in PCa tissue and there is positive correlation between MAPK1 and circSLC19A1. (A) Targetscan7.2 predicted the mutual binding sites between miR-326 and MAPK1. (B) Dual-luciferase reporter assay confirmed the interaction between miR-326 and MAPK1. (C) MAPK1 expression in DU145 and PC3 cells transfected with miR-326 inhibitor or inhibitor control was assessed by qRT-PCR, with GAPDH serving as a reference gene. (D) MAPK1 expression in PCa tissues and the adjacent normal tissue tissues was assessed by qRT-PCR, with GAPDH serving as a reference gene. (E) Pearson’s correlation analysis reflected the correlation between circSLC19A1 and MAPK1. (F) Pearson’s correlation analysis reflected the correlation between miR-326 and MAPK1. **P <0.01 and ***P<0.001 vs IC; ^###P<0.001 vs ANT.

Abbreviations: PCa, prostate cancer; IC, inhibitor control; I, miR-326 inhibitor; WT, wild type; MUT, mutant type; ANT, adjacent normal tissue; qRT-PCR, quantitative reverse-transcription polymerase chain reaction.