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. 2020 Nov 19;12:11883–11895. doi: 10.2147/CMAR.S267927

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© 2020 Huang et al.

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Silencing circSLC19A1 expression inhibited viability and proliferation in PCa cells by regulating miR-326/MAPK1 axis. (A) MAPK1 expression in DU145 and PC3 cells transfected with miR-326 inhibitor, sicircSLC19A1, siMAPK1 or miR-326 inhibitor plus sicircSLC19A1 was assessed by qRT-PCR, with GAPDH serving as a reference gene. (B) The viability of DU145 and PC3 cells transfected with miR-326 inhibitor, sicircSLC19A1, siMAPK1 or miR-326 inhibitor plus sicircSLC19A1 was measured by Cell Counting Kit-8 assay. (C) Representative photos of the colony formation of DU145 and PC3 cells transfected with miR-326 inhibitor, sicircSLC19A1, siMAPK1 or miR-326 inhibitor plus sicircSLC19A1. (D) The colony formation of DU145 and PC3 cells transfected with miR-326 inhibitor, sicircSLC19A1, siMAPK1 or miR-326 inhibitor plus sicircSLC19A1 was evaluated by cell colony formation assay. ***P<0.001 vs siNC+IC; ^{^^^}P<0.001 vs siNC+I; ^###P<0.001 vs sicirc+IC; ^ΔΔΔP<0.001 vs sicirc+I.

Abbreviations: sicircSLC19A1, siRNA-specific target circSLC19A1; siNC, siRNA-negative control; siMAPK1, siRNA-MAPK1; IC, inhibitor control; I, miR-326 inhibitor; qRT-PCR, quantitative reverse-transcription polymerase chain reaction.