REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human CD16-BUV395, Clone 3G8	BD	Cat #563785; RRID: AB_2744293
Anti-human CD15-BUV563, Clone W6D3	BD	Cat #741417; RRID: AB_2868406
Anti-human CD14-BUV615, Clone M5E2	BD	Cat #751150; RRID: AB_2875174
Anti-human CD56-BUV737, Clone NCAM16.2	BD	Cat #612767; RRID: AB_2860005
Anti-human CD31-CD31, Clone WM59	Biolegend	Cat #303123; RRID: AB_2562179
Anti-human HLADR-BV510, Clone L243	Biolegend	Cat #307646; RRID: AB_2561948
Anti-human CD11c-BV605, Clone 3.9	Biolegend	Cat #301635; RRID: AB_2562191
Anti-human CD3-BV650, Clone OKT3	Biolegend	Cat #317324; RRID: AB_2563352
Anti-human CD19-BV650, Clone HIB19	Biolegend	Cat #302237; RRID: AB_11126981
Anti-human CD10-BV711, Clone H110a	Biolegend	Cat #312226; RRID: AB_2565876
Anti-human CD123-BV785, Clone 6H6	Biolegend	Cat #306032; RRID: AB_2566448
Anti-human FCAR-FITC, Clone A59	Biolegend	Cat #354114; RRID: AB_2728341
Anti-human CD66b-PerCp Cy5.5, Clone G10F5	Biolegend	Cat #305108; RRID: AB_2077855
Anti-human CD9-PE, Clone HI9a	Biolegend	Cat #312106; RRID: AB_2075892
Anti-human CD163-PE-Cy7, Clone GHI/61	Biolegend	Cat #333614; RRID: AB_2562641
Anti-human CD41-APC, Clone HIP8	Biolegend	Cat #303710; RRID: AB_2249385
Anti-human CD304-APC-Fire, Clone 12C2	Biolegend	Cat #354523; RRID: AB_2715882
Anti-human CD19-FITC, Clone HIB19	BD	Cat #555412; RRID: AB_395812
Anti-human CD20-P5.5, Clone 2H7	BD	Cat #560736; RRID: AB_1727451
Anti-human CD3-BV711, Clone UCHT1	BD	Cat #563725; RRID: AB_2744392
Anti-human CD14-BV711, Clone MɸP9	BD	Cat #563372; RRID: AB_2744290
Anti-human CD16-BV711, Clone 3G8	BD	Cat #563127; RRID: AB_2732050
Anti-human IgM-BV510, Clone MHM-88	BioLegend	Cat #314521; RRID: AB_2561513
Anti-human IgD-PE-Cy7, Clone IA6-2	BD	Cat #561314; RRID: AB_10642457
Anti-human IgG-BV786, Clone G18-145	BD	Cat #564230; RRID: AB_2738684
Anti-human CD21-SB600 Clone, HB5	ThermoFisher	Cat #63-0219-41
Anti-human CD27-BV421, Clone M-T271	BioLegend	Cat #356418; RRID: AB_2562599
Anti-human CD38-AF700, Clone HIT2	ThermoFisher	Cat #56-0381-82; RRID: AB_657740
Anti-human IgA-Biotin, Clone IS11-8E10	Miltenyi	Cat #130-113-474; RRID: AB_2733547
Anti-human CD27-BV711, Clone M-T271	BioLegend	Cat #356430
Anti-human FCLR5-EF660, Clone 509F6	ThermoFisher	Cat #50-3078-42; RRID: AB_2574199
Anti-human CD11c-PEDazzle594, Clone 3.9	BioLegend	Cat #301642; RRID: AB_2564083
Anti-human Tbet-BV421, Clone 4B10	BioLegend	Cat #644816; RRID: AB_10959653
Anti-human Ki67-BUV395, Clone B56	BD	Cat #564071; RRID: AB_2738577
Anti-human CD45RA-BUV737, Clone HI100	BD	Cat #612846; RRID: AB_2870168
Anti-human CXCR5-BV421, Clone RF8B2	BD	Cat #562747; RRID: AB_2737766
Anti-human CD3-BV510, Clone UCHT1	BD	Cat #563109; RRID: AB_2732053
Anti-human CD4-BV605, Clone SK3	BioLegend	Cat #344646; RRID: AB_2734348
Anti-human CCR6-BV650, Clone 11A9	BD	Cat #563922; RRID: AB_2738488
Anti-human CD19-BV711, Clone SJ25C1	BD	Cat #563038; RRID: AB_2737970
Anti-human CD14-BV711, Clone M5E2	BD	Cat #740773; RRID: AB_2740436
Anti-human CD8-BV750, Clone SK1	BD	Cat #747097; RRID: AB_2871850
Anti-human CCR7-BV785, Clone G043H7	BioLegend	Cat #353230; RRID: AB_2563630
Anti-human FoxP3-AF488, Clone 259D/C7	BD	Cat #560047; RRID: AB_1645349
Anti-human ICOS-Percp-eF710, Clone ISA-3	ThermoFisher	Cat #46-9948-42; RRID: AB_10854730
Anti-human Bcl-6-PE, Clone 7D1	BioLegend	Cat #358504; RRID: AB_2562152
Anti-human Blimp-1-PE CF594, Clone 6D3	BD	Cat #565274; RRID: AB_2739147
Anti-human CXCR3-PE Cy5, Clone IC6/CXCR3	BD	Cat #561731; RRID: AB_10892799
Anti-human T-bet-PE Cy7, Clone 4B10	BioLegend	Cat #644823; RRID: AB_2561760
Anti-human PD-1-APC, Clone EH12.2H7	BioLegend	Cat #329908; RRID: AB_940475
Anti-human HLA-DR-APC H7, Clone L243	BioLegend	Cat #307610; RRID: AB_314688
Anti-human CD3-BUV395, Clone SK7	BD	Cat #564001; RRID: AB_2744382
Anti-human CXCR3-BV421, Clone BVD2-21C11	BD	Cat #562930; RRID: AB_2737899
Anti-human CCR7-BV605, Clone G043H7	BioLegend	Cat #353224; RRID: AB_2561753
Anti-human CD4-BV711, Clone OKT4	BioLegend	Cat #317440; RRID: AB_2562912
Anti-human CD127-BV786, Clone hIL7Rm21	BD	Cat #563324; RRID: AB_2738138
Anti-human pan-gdTCR-PerCP/ef710, Clone B1	BioLegend	Cat #331224; RRID: AB_2738871
Anti-human CCR4-PE/Dazzle594, Clone L291H4	BioLegend	Cat #359420; RRID: AB_2564095
Anti-human CD25-PE/Cy5, Clone BC96	BioLegend	Cat #302608; RRID: AB_314278
Anti-human CD8a-PE/Cy7, Clone SK1	ThermoFisher	Cat #25-0087-42; RRID: AB_10733019
Anti-human CD19-Af700, Clone SJ25C1	ThermoFisher	Cat #56-0198-42; RRID: AB_2815226
Anti-human CD8a-BV510, Clone SK1	BioLegend	Cat #344732; RRID: AB_2564624
Anti-human CD19-BV605, Clone SJ25C1	BioLegend	Cat #363006; RRID: AB_2564128
Anti-human IL-4-FITC, Clone MP4-25D2	BioLegend	Cat #500806; RRID: AB_315125
Anti-human IL-13-PE, Clone JES10-5A2	BioLegend	Cat #501903; RRID: AB_315198
Anti-human IL17a-PE/Dazzle594, Clone BL168	BioLegend	Cat #512336; RRID: AB_2564038
Anti-human IL-22-PE/Cy7, Clone 22URTI	ThermoFisher	Cat #25-7229-42; RRID: AB_10853346
Anti-human IFNg-Af700, Clone 4S.B3	BioLegend	Cat #502520; RRID: AB_528921
Anti-human pan-gdTCR-APC/Fire75, Clone B1	BioLegend	Cat #331228; RRID: AB_2650627
Anti-human CCR6-BV650, Clone OKT4	BioLegend	Cat #317436; RRID: AB_2563050
Anti-human CXCR5-BB515, Clone RF8B2	BD	Cat #564624; RRID: AB_2738871
Anti-human CCR10-PE, Clone 14305	R&D	Cat #FAB3478P-100
Anti-human CCR4-PE/Dazzle594, Clone L291H4	BioLegend	Cat #359420; RRID: AB_2564095
Anti-human CD25-PE/Cy5, Clone BC96	BioLegend	Cat #302608; RRID: AB_314278
Anti-human CD127-PE/Cy7, Clone hIL7Rm21	BD	Cat #560822; RRID: AB_2033938
Anti-human CD69-BUV395, Clone FN50	BD	Cat #564364; RRID: AB_2738770
Anti-human CD40L-FITC, Clone 24-31	ThermoFisher	Cat #11-1548-42; RRID: AB_10669047
Anti-human ICOS-Percp-eF710, Clone ISA-3	ThermoFisher	Cat #46-9948-42; RRID: AB_10854730
Anti-human IL-21-PE, Clone eBio3A3-N2	ThermoFisher	Cat #12-7219-42; RRID: AB_1582260
Anti-human IL-17A-PE Dz594, Clone BL168	Biolegend	Cat #512336; RRID: AB_2564038
Anti-human IL-4-PE Cy7, Clone MP4-25D2	BioLegend	Cat #500824; RRID: AB_2126746
Anti-human IL-6-AF647, Clone MQ2-13A5	BioLegend	Cat #501124; RRID: AB_2810624
Anti-human IL-2-AF700, Clone MQ1-17H12	BioLegend	Cat #500320; RRID: AB_528929
Anti-human IFNg-APC H7, Clone 4S.B3	BioLegend	Cat #502530; RRID: AB_10663412
Anti-mouse CD4-FITC, Clone GK1.5	BD	Cat #553729; RRID: AB_395013
Anti-mouse CD8-FITC, Clone 53.67	BD	Cat #553030; RRID: AB_394568
Anti-mouse B220-BV711, Clone RA3-6B2	BD	Cat #563892; RRID: AB_2738470
Anti-mouse CD138-BV605, Clone 281-2	BD	Cat #563147; RRID: AB_2721029
Anti-mouse CD38-AF700, Clone 90	ThermoFisher	Cat #56-0381-82; RRID: AB_657740
Anti-mouse Gl7-ef450, Clone GL-7	ThermoFisher	Cat #48-5902-82; RRID: AB_10870775
Anti-human IgG-HRP	Jackson ImmunoResearch	Cat #109-035-088; RRID: AB_2337584
anti-human IgM-HRP	Southern Biotech	Cat #1020-05; RRID: AB_2794201
anti-human IgA-HRP	Southern Biotech	Cat #2050-05; RRID: AB_2687526
Bacterial and Virus Strains
SARS-CoV-2 WA-1	BEI resources	NR-52281
Biological Samples
Human PBMC	This paper	N/A
Chemicals, Peptides, and Recombinant Proteins
Live/Dead Blue-BUV450	Thermo Fisher	Cat#L34962
Live/dead-ef780	ThermoFisher	Cat#65-0865-14; RRID: AB_2869553
Streptavidin-BUV395	BD	Cat#564176; RRID: AB_2869553
Decoy Tetreamer- PE-Cy5	This paper	N/A
RBD Tetramer- PE	This paper	N/A
PEI-MAX	Polyscience	Cat#26406
PDADMAC	Sigma Aldrich	Cat#409014
Talon cobalt affinity resin	Takara	Cat#635506
EZ-Link Sulfo-NHS-LC Biotinylation Kit	ThermoFisher	Cat#21435
SA-PE	Agilent	Cat#PJRS301-1
Alexa Fluor 647 Antibody Labeling Kit	ThermoFisher	Cat#A20186
1X 3,3′,5,5′-Tetramethylbenzidine (TMB)	Invitrogen	Cat#00-4201-56
Avicel RC-591	FMC
anti-PE magnetic beads	Miltenyi Biotec	Cat#130-048-801
eBioscience FoxP3 Fix/Perm kit	ThermoFisher	Cat#00-5521-00
Recombinant Spike Protein	(Walls et al., 2020)	N/A
Recombinant RBD Protein	(Walls et al., 2020)	N/A
phorbol 12-myristate 13-acetate	Sigma-Aldrich	Cat#P8139
Ionomycin	Sigma-Aldrich	Cat#I9657
GolgiStop/monensin	Becton Dickinson	Cat#554724
Cytofix/Cytoperm	Becton Dickinson	Cat#554714
Brefeldin A	Sigma-Aldrich	Cat#B6542
Cell Proliferation Dye eFluor 670	ThermoFisher	Cat#65-0840-85
recombinant human IL-2	Biolegend	Cat#589104
DreamTaq	Thermo Fisher	Cat#EP0702
FastAP	Thermo Fisher	Cat#EF0651
Exonuclease I	ThermoFisher	Cat#EN0582
Nutridoma-SP	Sigma-Aldrich	Cat#11011375001
Critical Commercial Assays
Human IgG ELISA Kit	Stemcell	Cat#01994
SMART-Seq v4	Takara	Cat#634470
In-Fusion Cloning Kit	Takara	Cat#638911
Experimental Models: Cell Lines
293T	ATCC	Cat#ACS-4500
Oligonucleotides
5′-GGAAGGAAGTCCTGTGCGAGGC-3′, 5′-GGAAGAAGCCCTGGACCAGGC-3′	(Wardemann and Busse, 2019)	N/A
5′-TCTTGTCCACCTTGGTGTTGCT’-3′	(Smith, 2009)	N/A
5′-GTTTCTCGTAGTCTGCTTTGCTCA-3′	(Smith, 2009)	N/A
5′-CACCAGTGTGGCCTTGTTGGCTTG-3′	(Smith, 2009)	N/A
5′-GTGGTATCAACGCAGAGTACATGGG-3′	This paper	N/A
Recombinant DNA
SARS-CoV-2 SB	BEI Resources	BEI NR-52422
SARS-CoV-2 S-2P ectodomain trimer	BEI Resources	GenBank: YP_009724390.1, BEI NR-52420; cite PMID 32155444
IgG1 vector	Smith et al., 2009	N/A
IgK vector	Smith et al., 2009	N/A
IgL vector	Smith et al., 2009	N/A
Software and Algorithms
FlowJo10	Becton Dickinson	N/A
Prism	GraphPad	N/A
Geneious Prime	Geneious	N/A