Fig. 4.

Repeated mild injuries induce tau mislocalization similar to a single severe injury. (A) Representative images of tau mislocalization in neurons exposed to periodic stretches of ε = 2%, $\dot{\epsilon }=\mathrm{1,000}\%/\mathrm{s}$. (B) Tau mislocalization, measured as fraction of spines with tau present, as an effect of number of stretches. Spines/images: control, 299/9; 1x, 384/6; 5x, 277/6; 10x, 241/5; 20x, 437/5. (C) Spine density as an effect of number of stretches. (D) Additive work predicted by the viscoelastic model for repeated stretches compared with experimentally measured tau mislocalization. In all plots, mean ± SD, *P < 0.05 compared to control, and NS is not significant.