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. 2020 Nov 2;117(46):29080–29089. doi: 10.1073/pnas.2015635117

Fig. 5.

Fig. 5.

Dissociable roles of divergent coeruleo-cortical projections in attentional control. (A) Schematic representation of the modified version of the of the two-choice task with distractors on a subset of trials. (B) Single-trial structure of the task. (C) Approach used for the expression of ChR2 in coeruleo-cortical terminals and their stimulation (5 Hz). (D) Confocal tile scan showing ChR2-eYFP (green) expression in LC neurons. (Scale bar, 500 µm.) (E) Magnified view of LC neurons expressing ChR2. (Scale bar, 50 µm.) (F) Histological samples showing LC terminals (NET; red) expressing ChR2 (eYFP; green) in the dmPFC (Left) and the vlOFC (Right). (G) Histological sample showing optic fiber placement (dashed lines) in the dmPFC (solid line). (Scale bar, 400 µm.) Effect of LC terminal stimulation in the dmPFC (n = 7) on correct responses (H), premature responses (I), and RTs (J). (K) Histological sample showing optic fiber placement (dashed lines) in vlOFC (solid line). (Scale bar, 400 µm.) Effect of LC terminal stimulation in vlOFC (n = 8) on correct responses (L), premature responses (M), and RTs (N). Data are presented as the mean of three sessions (150 trials/subject). Two-way RM ANOVA followed by Sidak post hoc test. AP: anterior-posterior coordinates. Effects of laser: n.s., not significant; *P < 0.05; **P < 0.005; ***P < 0.0005.