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. 2020 Nov 5;8:552020. doi: 10.3389/fcell.2020.552020

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Copyright © 2020 Tan, Yan, Li, Liang, Yu, Li, He, Li and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Low expression of lncRNA GAS5 attenuated the protective effect of CGA against ST invasion to MODE-K cells. GAS5 expression was knocked down in MODE-K cells, and then the toxic effect of CGA on cells was observed. (A) RT-qPCR was used to detect the expression of lncRNA GAS5; (B) gentamicin protection assay was used to detect the invasion rate of ST to MODE-K cells after different treatments; (C) RT-qPCR was used to detect IL-4, IL-6, TNF-α, and IL-1β levels; (D) ELISA detected IL-4, IL-6, TNF-α, and IL-1β levels. All experiments were repeated 3 times; The data in (A,B) were analyzed using one-way ANOVA and Tukey’s multiple comparison test; data in (C,D) were analyzed using two-way ANOVA and Tukey’s multiple comparison test; compared with ST + CGA group, **p < 0.01.