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. 2020 Nov 5;8:552020. doi: 10.3389/fcell.2020.552020

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Copyright © 2020 Tan, Yan, Li, Liang, Yu, Li, He, Li and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Low expression of miR-23a reversed the promoting effect of low expression of lncRNA GAS5 on the invasion to intestinal epithelial cells by ST. ST-infected MODE-K cells lowly expressing GAS5 were treated with low miR-23a expression to observe the protective effect of CGA on the cells. (A) Gentamicin protection assay was used to detect the invasion rate of ST after different CGA treatments; (B) RT-qPCR was used to detect the mRNA levels of IL-4, IL-6, TNF-α, and IL-1β; (C) ELISA was used to detect the levels of IL-4, IL-6, TNF-α, and IL-1β. All the experiments were repeated 3 times. The data in (A,B) were analyzed using t-test, and data in (C) were analyzed using two-way ANOVA and Tukey’s multiple comparison test. Compared with sh-GAS5 group, **p < 0.01, ^##p < 0.01.