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. 2020 Nov 5;8:552020. doi: 10.3389/fcell.2020.552020

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Copyright © 2020 Tan, Yan, Li, Liang, Yu, Li, He, Li and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CGA regulated the p38 MAPK signaling pathway through the lncRNAGAS5/miR-23a/PTEN axis to promote autophagy in ST infection. Effect of different concentrations of CGA on autophagy of MODE-K cells infected by ST was discussed. (A) Western blot analysis was used to detect the expression of p38 MAPK signaling pathway-related proteins in tissue and cells; (B) autophagosomes and colonies were observed by TEM (×5,000); (C,D) MDC fluorescence staining was used to detect cellular autophagy in each group; (D) the relative expression in MDC-positive cells; (E) Western blot detected the expression of autophagy-related proteins P62 and LC3II/LC3I. All the experiments were repeated 3 times. The data in (A,D,E) were analyzed using one-way ANOVA and Tukey’s multiple comparison test. Compared with Blank group, ST + CGA group or Sty + CGA group, *p < 0.05, **p < 0.01.